FixNCut: A Practical Guide to Sample Preservation by Reversible Fixation for Single Cell Assays.

ABSTRACT: The quality of standard single-cell experiments often depends on the immediate processing of cells or tissues post-harvest to preserve fragile and vulnerable cell populations, unless the samples are adequately fixed and stored. Despite the recent rise in popularity of probe-based and aldehyde-fixed RNA assays, these methods face limitations in species and target availability and are not suitable for immunoprofiling or assessing chromatin accessibility. Recently, a reversible fixation strategy known as FixNCut has been successfully deployed to separate sampling from downstream applications in a reproducible and robust manner, avoiding stress or necrosis-related artifacts. In this article, we present an optimized and robust practical guide to the FixNCut protocol to aid the end-to-end adapta

SUBMITTER: Wang S 

PROVIDER: S-EPMC11417608 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature