Low-Volume <i>Ex Situ</i> Lung Perfusion System for Single Lung Application in a Small Animal Model Enables Optimal Compliance With "<i>Reduction</i>" in 3R Principles of Animal Research.

ABSTRACT: Ex situ lung perfusion (ESLP) is used for organ reconditioning, repair, and re-evaluation prior to transplantation. Since valid preclinical animal models are required for translationally relevant studies, we developed a 17 mL low-volume ESLP for double- and single-lung application that enables cost-effective optimal compliance "reduction" of the 3R principles of animal research. In single-lung mode, ten Fischer344 and Lewis rat lungs were subjected to ESLP and static cold storage using STEEN or PerfadexPlus. Key perfusion parameters, thermal lung imaging, blood gas analysis (BGA), colloid oncotic pressure (COP), lung weight gain, histological work-up, and cytokine analysis were performed. Significant differences between perfusion solutions but not between the rat strains were detect

SUBMITTER: Katsirntaki K 

PROVIDER: S-EPMC11418019 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature