A high-resolution ¹³C NMR approach for profiling fatty acid unsaturation in lipid extracts and in live Caenorhabditiselegans.

ABSTRACT: Unsaturated fatty acids (UFA) play a crucial role in central cellular processes in animals, including membrane function, development, and disease. Disruptions in UFA homeostasis can contribute to the onset of metabolic, cardiovascular, and neurodegenerative disorders. Consequently, there is a high demand for analytical techniques to study lipid compositions in live cells and multicellular organisms. Conventional analysis of UFA compositions in cells, tissues, and organisms involves solvent extraction procedures coupled with analytical techniques such as gas chromatography, MS and/or NMR spectroscopy. As a nondestructive and nontargeted technique, NMR spectroscopy is uniquely capable of characterizing the chemical profiling of living cells and multicellular organisms. Here, we use NMR spect

SUBMITTER: Cravero BH 

PROVIDER: S-EPMC11418130 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature