CXCR4-enriched T regulatory cells preferentially home to bone marrow and resolve inflammation.

ABSTRACT: CXCR4 cell surface expression is critical for the homing of T regulatory (Treg) cells to the bone marrow (BM). We hypothesize that CXCR4 enrichment on Tregs cell surface may abbreviate their transit time to reach BM. Umbilical cord-blood CD25⁺ Tregs underwent CXCR4 dual enrichment and ex vivo expansion using the CRANE process to generate CXCR4-enriched Tregs (Treg^CXCR4) cells, which showed a faster migration across the Transwell membrane toward CXCL12/stromal cell-derived factor 1α (SDF1α) at 15, 30, and 60 min, when compared to unmanipulated Treg^control cells (p < 0.0001). Treg^CXCR4 exhibited preferential homing to BM in vivo at 12 and 24 h. Metacluster analysis of BM showed a decrease in CD8⁺ and an increase in C

SUBMITTER: Huang M 

PROVIDER: S-EPMC11418154 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature