Spermatozoa from male mice with infertility due to Odf4 deficiency can fertilize oocytes by in vitro fertilization.

ABSTRACT:

Purpose

The purpose of this study is to confirm whether in vitro fertilization (IVF) with spermatozoa from Odf4-deficient infertile males (Odf4 ^-/- spermatozoa) can lead to the development of zygotes, which was reported in a previous in vivo study.

Methods

In vitro capacitation and IVF were performed using Odf4 ^-/- spermatozoa in a small drop of TYH medium with pyruvate and glucose, for 60 min or up to 4 days. A capacitation test was performed by immunoblotting using an anti-p-Tyr antibody. A sperm movement test was performed using a computer-assisted sperm motility analysis system (SMAS). An IVF fertilization test was also performed to evaluate zygote production. Videos were taken by a DMi8 stereomicroscope equipped with a high-spe

SUBMITTER: Ito C 

PROVIDER: S-EPMC11418632 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature