Hypoxia and loss of <i>GCM1</i> expression prevents differentiation and contact inhibition in human trophoblast stem cells.

ABSTRACT: The placenta develops alongside the embryo and nurtures fetal development to term. During the first stages of embryonic development, due to low blood circulation, the blood and ambient oxygen supply is very low (~1-2% O₂) and gradually increases upon placental invasion. While a hypoxic environment is associated with stem cell self-renewal and proliferation, persistent hypoxia may have severe effects on differentiating cells and could be the underlying cause of placental disorders. We find that human trophoblast stem cells (hTSC) thrive in low oxygen, whereas differentiation of hTSC to trophoblast to syncytiotrophoblast (STB) and extravillous trophoblast (EVT) is negatively affected by hypoxic conditions. The pro-differentiation factor GCM1 (human Glial Cell Missing-1) is downreg

SUBMITTER: Cinkornpumin JK 

PROVIDER: S-EPMC11419009 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature