The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.

ABSTRACT: The cilium is a microtubule-based organelle critical for many cellular functions. Its assembly initiates at a basal body and continues as an axoneme that projects out of the cell to form a functional cilium. This assembly process is tightly regulated. However, our knowledge of the molecular architecture and the mechanism of assembly is limited. By applying electron cryotomography and subtomogram averaging, we obtained subnanometer resolution structures of the inner junction in three distinct regions of the cilium: the proximal region of the basal body, the central core of the basal body, and the flagellar axoneme. The structures allowed us to identify several basal body and axoneme components. While a few proteins are distributed throughout the entire length of the organelle, many are rest

SUBMITTER: Li S 

PROVIDER: S-EPMC11419100 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature