NuRD chromatin remodeling is required to repair exogenous DSBs in the <i>Caenorhabditis elegans</i> germline.

ABSTRACT: Organisms rely on coordinated networks of DNA repair pathways to protect genomes against toxic double-strand breaks (DSBs), particularly in germ cells. All repair mechanisms must successfully negotiate the local chromatin environment in order to access DNA. For example, nucleosomes can be repositioned by the highly conserved Nucleosome Remodeling and Deacetylase (NuRD) complex. In Caenorhabditis elegans, NuRD functions in the germline to repair DSBs - the loss of NuRD's ATPase subunit, LET-418/CHD4, prevents DSB resolution and therefore reduces fertility. In this study, we challenge germlines with exogenous DNA damage to better understand NuRD's role in repairing DSBs. We find that let-418 mutants are sensitive to cisplatin and hydroxyurea: exposure to either mutagen impedes

SUBMITTER: Ananthaswamy D 

PROVIDER: S-EPMC11419128 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature