Detection of enterovirus RNA in pancreas and lymphoid tissues of organ donors with type 1 diabetes.

ABSTRACT:

Aims/hypothesis

The nPOD-Virus group collaboratively applied innovative technologies to detect and sequence viral RNA in pancreas and other tissues from organ donors with type 1 diabetes. These analyses involved the largest number of pancreas samples collected to date.

Methods

We analysed pancreas, spleen, pancreatic lymph nodes, and duodenum samples from the following donor groups: a) donors with type 1 diabetes (n=71), with (n=35) or without (n=36) insulin-containing islets, (b) donors with single or double islet autoantibody positivity without diabetes (n=22) and c) autoantibody-negative donors without diabetes (control donors) (n=74). Five research laboratories participated in this collaborative effort using approaches for unbiased discovery of RNA viruses (two RNA-Seq p

SUBMITTER: Laiho JE 

PROVIDER: S-EPMC11419248 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature