Matrix metalloproteinases mediate influenza A-associated shedding of the alveolar epithelial glycocalyx.

ABSTRACT:

Background

The alveolar epithelium is protected by a heparan sulfate-rich, glycosaminoglycan layer called the epithelial glycocalyx. It is cleaved in patients with acute respiratory distress syndrome (ARDS) and in murine models of influenza A (IAV) infection, shedding fragments into the airspace from the cell surface. Glycocalyx shedding results in increased permeability of the alveolar-capillary barrier, amplifying acute lung injury. The mechanisms underlying alveolar epithelial glycocalyx shedding in IAV infection are unknown. We hypothesized that induction of host sheddases such as matrix metalloproteinases (MMPs) during IAV infection results in glycocalyx shedding and increased lung injury.

Materials and methods

We measured glycocalyx shedding and lung injury during IAV

SUBMITTER: Schaaf KR 

PROVIDER: S-EPMC11419339 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature