Protocol for volume correlative light X-ray and electron microscopy of endothelial cells in mouse tissue.

ABSTRACT: Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al.¹.

SUBMITTER: Reglero-Real N 

PROVIDER: S-EPMC11419916 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature