Biofabrication of prevascularized spheroids for bone tissue engineering by fusion of microvascular fragments with osteoblasts.

ABSTRACT:

Introduction

Spheroids are promising building blocks for scaffold-free bone tissue engineering. Their rapid vascularization is of major importance to guarantee their survival after transplantation. To achieve this, we herein introduce the biofabrication of prevascularized spheroids by fusion of adipose tissue-derived microvascular fragments (MVF) with osteoblasts (OB).

Methods

For this purpose, 200 MVF from donor mice and 5,000, 10,000 or 20,000 murine OB (MC3T3-E1) were co-cultured in a liquid overlay system for 3 days to generate OB + MVF spheroids. OB mono-culture spheroids served as controls.

Results and discussion

During the generation process, the diameters of all spheroids progressively decreased, resulting in compact, viable spheroids of homogeneous sizes. MVF

SUBMITTER: Wrublewsky S 

PROVIDER: S-EPMC11419975 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature