Trans-eyelid distribution of epinastine to the conjunctiva following eyelid application in rabbits.

ABSTRACT:

Purpose

To reveal the penetration of epinastine, an anti-allergic ophthalmic agent, into the eyelid and its distribution to the conjunctiva after administration of a cream formulation on rabbit eyelid skin.

Study design

Experimental study.

Methods

Rabbits were treated with 0.5% epinastine cream on hair-shaved eyelids, followed by preparation of eyelid tissue slices to determine spatial tissue distribution of epinastine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) quantification using laser-microdissected tissues and desorption electrospray ionization mass spectrometry imaging (DESI-MSI). In addition, following either eyelid application of 0.5% epinastine cream or ocular instillation of 0.1% epinastine eye drops, concentration-time profiles of epinastin

SUBMITTER: Mochizuki T 

PROVIDER: S-EPMC11420250 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature