Impact of singular versus combinatorial environmental stress on RONS generation in Drosophila melanogaster larvae.

ABSTRACT: We investigated environmentally correlated abiotic stressor desiccation (D), heat (H), and starvation (S) in the generation of reactive oxygen and nitrogen species (RONS) using Drosophila melanogaster larvae as an experimental model, subjected to either individual stressors or exposed to a combinatorial form of stressors (D + H, H + S, and D + S). The study was also extended to find synergistic endpoints where the impacts of all three stressors (D + H + S) were exerted simultaneously. We estimated the lethal time (LT₂₀) at specific doses using regression and probit analyses based on the larval survival. LT₂₀ values were used as the base-level parameter for further oxidative stress experimental analysis work. First, all stressors led to the activation of a typic

SUBMITTER: Bomble P 

PROVIDER: S-EPMC11420561 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature