A comparative genomic and phenotypic study of <i>Vibrio cholerae</i> model strains using hybrid sequencing.

ABSTRACT: Next-generation sequencing methods have become essential for studying bacterial biology and pathogenesis, often depending on high-quality, closed genomes. In this study, we utilized a hybrid sequencing approach to assemble the genome of C6706, a widely used Vibrio cholerae model strain. We present a manually curated annotation of the genome, enhancing user accessibility by linking each coding sequence to its counterpart in N16961, the first sequenced V. cholerae isolate and a commonly used reference genome. Comparative genomic analysis between V. cholerae C6706 and N16961 uncovered multiple genetic differences in genes associated with key biological functions. To determine whether these genetic variations result in phenotypic differences, we compared several phenotypes

SUBMITTER: Lorentzen OM 

PROVIDER: S-EPMC11420891 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature