Branched Polymeric Prenucleation Assemblies Initiate Calcium Phosphate Precipitation.

ABSTRACT: The formation of crystalline calcium phosphate (CaP) has recently gained ample attention as it does not follow the classic nucleation-and-growth mechanism of solid formation. Instead, the precipitation mechanisms can involve numerous intermediates, including soluble prenucleation species. However, structural features, stability, and transformation of such solution-state precursors remain largely undisclosed. Herein, we report a detailed and comprehensive characterization of the sequential events involved in calcium phosphate crystallization starting from the very early prenucleation stage. We integrated an extensive set of time-resolved methods, including NMR, turbidimetry, SAXS, cryo-TEM, and calcium-potentiometry to show that CaP nucleation is initiated by the transformation of "branched

SUBMITTER: Turhan E 

PROVIDER: S-EPMC11421018 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature