Influence of in vivo growth on human glioma cell line gene expression: convergent profiles under orthotopic conditions.

ABSTRACT: Defining the molecules that regulate tumor cell survival is an essential prerequisite for the development of targeted approaches to cancer treatment. Whereas many studies aimed at identifying such targets use human tumor cells grown in vitro or as s.c. xenografts, it is unclear whether such experimental models replicate the phenotype of the in situ tumor cell. To begin addressing this issue, we have used microarray analysis to define the gene expression profile of two human glioma cell lines (U251 and U87) when grown in vitro and in vivo as s.c. or as intracerebral (i.c.) xenografts. For each cell line, the gene expression profile generated from tissue culture was significantly different from that generated from the s.c. tumor, which was significantly different from those grown i.c. The di

SUBMITTER: Camphausen K 

PROVIDER: S-EPMC1142119 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature