Fusion of FokI and catalytically inactive prokaryotic Argonautes enables site-specific programmable DNA cleavage.

ABSTRACT: Site-specific nucleases are crucial for genome engineering applications in medicine and agriculture. The ideal site-specific nucleases are easily reprogrammable, highly specific in target site recognition, and robust in nuclease activities. Prokaryotic Argonaute (pAgo) proteins have received much attention as biotechnological tools due to their ability to recognize specific target sequences without a protospacer adjacent motif, but their lack of intrinsic dsDNA unwinding activity limits their utility in key applications such as gene editing. Recently, we developed a pAgo-based system for site-specific DNA cleavage at physiological temperatures independently of the DNA form, using peptide nucleic acids (PNAs) to facilitate unwinding dsDNA targets. Here, we fused catalytically dead pAgos wit

SUBMITTER: Wang Q 

PROVIDER: S-EPMC11421335 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature