Supported gel slab scaffolds as a three-dimensional cell-based assay platform.

ABSTRACT: Cell-based assays are heavily relied on in the drug discovery pipeline, quickly pairing down large compound libraries to a manageable number of drug candidates for further characterization and evaluation. Monolayer cultures in which cells are deposited onto the bottom of well plates are the workhorse of many of these screens despite continued evidence of their inability to predict in vivo responses. Three-dimensional (3D) culture platforms can generate tissue-like environments with more representative cellular phenotypes than monolayers but have proven challenging to incorporate into already-developed workflows. Scaffold-based approaches are a tractable means of generating tissue-like environments, supporting cell-laden gels whose preparation is analogous to depositing cells in a we

SUBMITTER: Sitte ZR 

PROVIDER: S-EPMC11421879 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature