A hypothalamus-lateral periaqueductal gray GABAergic neural projection facilitates arousal following sevoflurane anesthesia in mice.

ABSTRACT:

Background

The lateral hypothalamus (LHA) is an evolutionarily conserved structure that regulates basic functions of an organism, particularly wakefulness. To clarify the function of LHA^GABA neurons and their projections on regulating general anesthesia is crucial for understanding the excitatory and inhibitory effects of anesthetics on the brain. The aim of the present study is to investigate whether LHA^GABA neurons play either an inhibitory or a facilitatory role in sevoflurane-induced anesthetic arousal regulation.

Methods

We used fiber photometry and immunofluorescence staining to monitor changes in neuronal activity during sevoflurane anesthesia. Opto-/chemogenetic modulations were employed to study the effect of neurocircuit modulations during the

SUBMITTER: Wang D 

PROVIDER: S-EPMC11421888 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature