Optogenetic Functional Activation Is Detrimental During Acute Ischemic Stroke in Mice.

ABSTRACT:

Background

Functional activation of the focal ischemic brain has been reported to improve outcomes by augmenting collateral blood flow. However, functional activation also increases metabolic demand and might thereby worsen outcomes. Indeed, preclinical and clinical reports have been conflicting. Here, we tested the effect of functional activation during acute ischemic stroke using distal middle cerebral artery occlusion in anesthetized mice.

Methods

Using transgenic mice expressing channelrhodopsin-2 in neurons, we delivered functional activation using physiological levels of transcranial optogenetic stimulation of the moderately ischemic cortex (ie, penumbra), identified using real-time full-field laser speckle perfusion imaging during a 1-hour distal microvascular clip of

SUBMITTER: Sugimoto K 

PROVIDER: S-EPMC11421960 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature