Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer.

ABSTRACT: While heterogeneity is a key feature of cancer, understanding metabolic heterogeneity at the single-cell level remains a challenge. Here we present ¹³C-SpaceM, a method for spatial single-cell isotope tracing that extends the previously published SpaceM method with detection of ¹³C₆-glucose-derived carbons in esterified fatty acids. We validated ¹³C-SpaceM on spatially heterogeneous models using liver cancer cells subjected to either normoxia-hypoxia or ATP citrate lyase depletion. This revealed substantial single-cell heterogeneity in labelling of the lipogenic acetyl-CoA pool and in relative fatty acid uptake versus synthesis hidden in bulk analyses. Analysing tumour-bearing brain tissue from mice fed a ¹³C₆-glucose-contai

SUBMITTER: Buglakova E 

PROVIDER: S-EPMC11422168 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature