Fast and quantitative mitophagy assessment by flow cytometry using the mito-QC reporter.

ABSTRACT: Mitochondrial quality control is finely tuned by mitophagy, the selective degradation of mitochondria through autophagy, and mitochondrial biogenesis. Removal of damaged mitochondria is essential to preserve cellular bioenergetics and prevent detrimental events such as sustained mitoROS production, pro-apoptotic cytochrome c release or mtDNA leakage. The array of tools available to study mitophagy is very limited but in constant development. Almost a decade ago, we developed a method to assess mitophagy flux using MitoTracker Deep Red in combination with lysosomal inhibitors. Now, using the novel tandem-fluorescence reporter mito-QC (mCherry-GFP-FIS1^101-152) that allows to differentiate between healthy mitochondria (mCherry⁺GFP⁺) and mitolysosomes (m

SUBMITTER: Jimenez-Loygorri JI 

PROVIDER: S-EPMC11422238 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature