Study on cellular uptake of a hydrophobic near-infrared-absorbing diradical-platinum(II) complex solubilized by albumin using hyperspectral imaging, spectrophotometry, and spectrofluorimetry.

ABSTRACT: Owing to its biopenetrability and minimal invasiveness, near-infrared (NIR) light in the region between 700-1100 nm has attracted attention in cancer diagnosis and therapy. Our group previously reported that the hydrophobic diradical-platinum(II) complex PtL₂ is a promising agent for cancer photothermal therapy (L = 3,5-dibromo-1,2-diiminobenzosemiquinonate radical). Because PtL₂ does not fluoresce, its intercellular uptake of PtL₂ cannot be observed with a fluorescence microscope. In this study, we clarified the uptake and intracellular behavior of PtL₂ solubilized by bovine serum albumin (BSA) using hyperspectral imaging enabling spectrophotometric analysis of the image. The spectral changes in the obtained images indicated that the internaliza

SUBMITTER: Sawamura R 

PROVIDER: S-EPMC11422251 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature