Identification and characterization of Lacticaseibacillus rhamnosus HP-B1083-derived β-glucuronidase and its application for baicalin biotransformation.

ABSTRACT: Baicalein, showing higher bioavailability and stronger pharmacological activity, can be obtained via a β-glucuronidase (GUS)-catalyzed transformation of baicalein 7-O-β-D-glucuronide (baicalin). Recently, we have found that the fermentation broth of Lacticaseibacillus rhamnosus HP-B1083 can efficiently convert baicalin to baicalein. In this study, the L. rhamnosus HP-B1083-derived enzyme involved in baicalin biotransformation was identified and characterized. First, the LruidA gene, encoding the responsible enzyme, was cloned and sequenced. Sequence analysis revealed that the deduced enzyme (designated as LrUidA) belonged to the glycosyl hydrolase family 2. The recombinant LrUidA was expressed and purified for characterization. LrUidA had a molecular weight of 70 kDa,

SUBMITTER: Ji XL 

PROVIDER: S-EPMC11422588 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature