START: A Versatile Platform for Bacterial Ligand Sensing with Programmable Performances.

ABSTRACT: Recognition of signaling molecules for coordinated regulation of target genes is a fundamental process for biological systems. Cells often rely on transcription factors to accomplish these intricate tasks, yet the subtle conformational changes of protein structures, coupled with the complexity of intertwined protein interaction networks, pose challenges for repurposing these for bioengineering applications. This study introduces a novel platform for ligand-responsive gene regulation, termed START (Synthetic Trans-Acting Riboswitch with Triggering RNA). Inspired by the bacterial ligand sensing system, riboswitch, and the synthetic gene regulator, toehold switch, the START platform enables the implementation of synthetic biosensors for various ligands. Rational sequence design with targeted

SUBMITTER: Kim J 

PROVIDER: S-EPMC11423158 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature