Programmable Bacteria with Dynamic Virulence Modulation System for Precision Antitumor Immunity.

ABSTRACT: Engineered bacteria-mediated antitumor approaches have been proposed as promising immunotherapies for cancer. However, the off-target bacterial toxicity narrows the therapeutic window. Living microbes will benefit from their controllable immunogenicity within tumors for safer antitumor applications. In this study, a genetically encoded microbial activation strategy is reported that uses tunable and dynamic expression of surface extracellular polysaccharides to improve bacterial biocompatibility while retaining therapeutic efficacy. Based on screening of genes associated with Salmonella survival in macrophages, a novel attenuated Salmonella chassis strain AIS (htrA gene-deficient) highly enriched in tumors after administration and rapidly cleared from normal organs are reported. Subsequentl

SUBMITTER: Wu L 

PROVIDER: S-EPMC11423194 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature