NeuroConstruct: 3D Reconstruction and Visualization of Neurites in Optical Microscopy Brain Images.

ABSTRACT: We introduce NeuroConstruct, a novel end-to-end application for the segmentation, registration, and visualization of brain volumes imaged using wide-field microscopy. NeuroConstruct offers a Segmentation Toolbox with various annotation helper functions that aid experts to effectively and precisely annotate micrometer resolution neurites. It also offers an automatic neurites segmentation using convolutional neuronal networks (CNN) trained by the Toolbox annotations and somas segmentation using thresholding. To visualize neurites in a given volume, NeuroConstruct offers a hybrid rendering by combining iso-surface rendering of high-confidence classified neurites, along with real-time rendering of raw volume using a 2D transfer function for voxel classification score versus voxel intensity val

SUBMITTER: Ghahremani P 

PROVIDER: S-EPMC11423259 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature