Plasma concentration of thrombopoietin in dogs with immune thrombocytopenia.

ABSTRACT:

Background

Immune thrombocytopenia (ITP) is a common cause of severe thrombocytopenia in dogs. The pathogenesis of nonassociative, primary ITP (pITP) appears complex, with ill-defined thrombopoietic response.

Objectives

Develop an immunoassay to measure plasma canine thrombopoietin (TPO) concentration and characterize TPO concentrations in dogs with pITP.

Animals

Forty-one healthy dogs, 8 dogs in an induced ITP model (3 control, 5 ITP), and 58 pITP dogs.

Methods

Recombinant canine TPO (rcTPO) was purchased and its identity confirmed by mass spectrometry. Monoclonal antibodies were raised to rcTPO and used to configure a sandwich ELISA using streptavidin-biotin detection. Assay performance, coefficients of variability, and healthy dog plasma TPO reference interv

SUBMITTER: Brooks MB 

PROVIDER: S-EPMC11423463 | biostudies-literature | 2024 Sep-Oct

REPOSITORIES: biostudies-literature