Metabolomic profiling of pheochromocytomas in dogs: Catecholamine phenotype and tricarboxylic acid cycle metabolites.

ABSTRACT:

Background

In humans with pheochromocytomas (PCCs), targeted metabolomics is used to determine the catecholamine phenotype or to uncover underlying pathogenic variants in tricarboxylic acid (TCA) cycle genes such as succinate dehydrogenase subunits (SDHx).

Hypothesis/objectives

To analyze catecholamine contents and TCA cycle metabolites of PCCs and normal adrenals (NAs).

Animals

Ten healthy dogs, 21 dogs with PCC.

Methods

Prospective observational study. Dogs diagnosed with PCC based on histopathological and immunohistochemical confirmation were included. Tissue catecholamine contents and TCA metabolites in PCCs and NAs were measured by liquid chromatography with mass spectrometry or electrochemical detection.

Results

Compared to NAs, PCCs had significan

SUBMITTER: van den Berg MF 

PROVIDER: S-EPMC11423489 | biostudies-literature | 2024 Sep-Oct

REPOSITORIES: biostudies-literature