Delivery of Yersinia pestis antigens via Escherichia coli outer membrane vesicles offered improved protection against plague.

ABSTRACT: Outer membrane vesicles (OMVs) from Gram-negative bacteria can be used as a vaccine platform to deliver heterologous antigens. Here, the major protective antigens of Yersinia pestis, F1 and LcrV, were fused either with the leader sequence or the transmembrane domain of the outer membrane protein A (OmpA), resulting in chimeric proteins OmpA-ls-F1V and OmpA_46-159-F1V, respectively. We show that OmpA-ls-F1V and OmpA_46-159-F1V can be successfully delivered into the lumen and membrane of the OMVs of Escherichia coli, respectively. Mutation of ompA but not tolR in E. coli enhanced the delivery efficiency of OmpA-ls-F1V into OMVs. The OmpA-ls-F1V protein comprises up to 20% of the total protein in OMVs derived from the ompA mutant (

SUBMITTER: Tong Z 

PROVIDER: S-EPMC11423571 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature