Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalyst Methylococcus capsulatus Bath.

ABSTRACT: Methanotrophic bacteria play a vital role in the biogeochemical carbon cycle due to their unique ability to use CH₄ as a carbon and energy source. Evidence suggests that some methanotrophs, including Methylococcus capsulatus, can also use CO₂ as a carbon source, making these bacteria promising candidates for developing biotechnologies targeting greenhouse gas capture and mitigation. However, a deeper understanding of the dual CH₄ and CO₂ metabolism is needed to guide methanotroph strain improvements and realize their industrial utility. In this study, we show that M. capsulatus expresses five carbonic anhydrase (CA) isoforms, one α-CA, one γ-CA, and three β-CAs, that play a role in its inorganic carbon metabolism and CO₂-

SUBMITTER: Lee SA 

PROVIDER: S-EPMC11423575 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature