Rapid and sensitive determination of residual prion infectivity from prion-decontaminated surfaces.

ABSTRACT: Prion diseases are untreatable fatal transmissible neurodegenerative diseases that affect a wide range of mammals, including humans, and are caused by PrP^Sc, the infectious self-templating conformation of the host-encoded protein, PrP^C. Prion diseases can be transmitted via surfaces (e.g., forceps, EEG electrodes) in laboratory and clinical settings. Here, we use a combination of surface swabbing and real-time quaking-induced conversion (RT-QuIC) to test for residual surface-associated prions following prion disinfection. We found that treatment of several prion-contaminated laboratory and clinically relevant surfaces with either water or 70% EtOH resulted in robust detection of surface-associated prions. In contrast, treatment of surfaces with sodium hypochlorite res

SUBMITTER: Simmons SM 

PROVIDER: S-EPMC11423590 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature