Role of glycogen metabolism in <i>Clostridioides difficile</i> virulence.

ABSTRACT: Glycogen plays a vital role as an energy reserve in various bacterial and fungal species. Clostridioides difficile possesses a glycogen metabolism operon that contains genes for both glycogen synthesis and utilization. In our investigation, we focused on understanding the significance of glycogen metabolism in the physiology and pathogenesis of C. difficile. To explore this, we engineered a C. difficile JIR8094 strain lacking glycogen synthesis capability by introducing a group II intron into the glgC gene, the operon's first component. Quantification of intracellular glycogen levels validated the impact of this modification. Interestingly, the mutant strain exhibited a 1.5-fold increase in toxin production compared with the parental strain, without significant

SUBMITTER: Hasan MK 

PROVIDER: S-EPMC11423593 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature