Loss of synovial tissue macrophage homeostasis precedes rheumatoid arthritis clinical onset.

ABSTRACT: This study performed an in-depth investigation into the myeloid cellular landscape in the synovium of patients with rheumatoid arthritis (RA), "individuals at risk" of RA, and healthy controls (HC). Flow cytometric analysis demonstrated the presence of a CD40-expressing CD206⁺CD163⁺ macrophage population dominating the inflamed RA synovium, associated with disease activity and treatment response. In-depth RNA sequencing and metabolic analysis demonstrated that this macrophage population is transcriptionally distinct, displaying unique inflammatory and tissue-resident gene signatures, has a stable bioenergetic profile, and regulates stromal cell responses. Single-cell RNA sequencing profiling of 67,908 RA and HC synovial tissue cells identified nine transcriptionally d

SUBMITTER: Hanlon MM 

PROVIDER: S-EPMC11423874 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature