OptoAssay-Light-controlled dynamic bioassay using optogenetic switches.

ABSTRACT: Circumventing the limitations of current bioassays, we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics. Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light. Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format. The applicability of the OptoAssay is successfully demonstrated by the calibration of a competitive model assay, resulting in a superior limit of detection of 8 pg ml^-1, which is beyond those of conventional ELISA tests. In the future, combined with smartphones, OptoA

SUBMITTER: Urban N 

PROVIDER: S-EPMC11423887 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature