Tracking the interactions of rRNA processing proteins during nucleolar assembly in living cells.

ABSTRACT: Reorganization of the nuclear machinery after mitosis is a fundamental but poorly understood process. Here, we investigate the recruitment of the nucleolar processing proteins in the nucleolus of living cells at the time of nucleus formation. We question the role of the prenucleolar bodies (PNBs), during migration of the processing proteins from the chromosome periphery to sites of rDNA transcription. Surprisingly, early and late processing proteins pass through the same PNBs as demonstrated by rapid two-color four-dimensional imaging and quantification, whereas a different order of processing protein recruitment into nucleoli is supported by differential sorting. Protein interactions along the recruitment pathway were investigated using a promising time-lapse analysis of fluorescence reso

SUBMITTER: Angelier N 

PROVIDER: S-EPMC1142430 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature