Specific cell subclusters of dental pulp stem cells respond to distinct pathogens through the ROS pathway.

ABSTRACT:

Introduction

Microbial pathogens invade various human organs, including the oral cavity. Candida albicans (C.a) and Streptococcus mutans (S.m) served respectively as representative oral pathogenic fungi and bacteria to stimulate dental pulp stem cells (DPSCs) and to screen the DPSC subcluster that specifically responded to fungal infection.

Methods

DPSCs were obtained from the impacted third molars of six healthy subjects. Then, cells were mixed and divided into three samples, two of which were stimulated with C.a and S.m, respectively; the third sample was exposed to cell medium only (Ctrl). Single-cell mRNA sequencing analysis of treated DPSCs was performed.

Results

DPSCs were composed of four major clusters of which one, DPSC.7, exhibited unique chang

SUBMITTER: Xu T 

PROVIDER: S-EPMC11424553 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature