Identification of differentially expressed miRNAs between male sterile and fertile floral buds in watermelon (<i>Citrullus lanatus</i> L.) via high-throughput sequencing.

ABSTRACT: This experiment used floral buds from watermelon genic male sterile dual-purpose lines as materials to explore the differentially expressed miRNAs (DEMs) between male fertile and sterile floral buds of watermelon. Paraffin sectioning technology was employed for a cytological analysis, and small RNA sequencing was used to explore miRNAs related to anther or pollen development. Cytological analysis indicated that the abnormal development of tapetal cells may cause microspore abortion. Small RNA sequencing identified a total of 314 miRNAs (29 known and 285 novel, which belonged to 12 and 61 miRNA families, respectively) in floral buds. Differential expression revealed 36 (5 known and 31 novel) DEMs between male fertile and sterile buds, 7 and 29 of which were up-regulated and down-regulated,

SUBMITTER: Zhang G 

PROVIDER: S-EPMC11424599 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature