A molecular standard for circulating HBV RNA detection and quantification assays in patients with chronic hepatitis B.

ABSTRACT:

Background & aims

Circulating HBV RNAs have been proposed as a biomarker that reflects the transcriptional activity of covalently closed circular DNA (cccDNA) and may help to evaluate HBV treatment activity. Different research assays have been proposed and, although two PCR-based research use only investigational assays have been developed, the lack of standardized protocols represents an important limitation. Here we have designed and generated a stable clonal cell line producing an RNA-based standard for the calibration of PCR-based circulating HBV RNA assays.

Methods

HBV RNA-producing Huh7-derived stable cell lines were generated by transfecting pTriEX plasmids containing 1.1 unit length HBV DNA genomes carrying mutations in the catalytic site (YMAA mutation) and the TP d

SUBMITTER: Paturel A 

PROVIDER: S-EPMC11424956 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature