Adenosine Deaminase-Like Gene-Carried Lentivirus Toolkit for Identification of DNA N⁶-Methyladenine Origins.

ABSTRACT: Post-replicative DNA N⁶-methyladenine (pr6mdA) can form via bona fide methylase-catalyzed adenine methylation, playing a pivotal role in embryonic development and other biological processes. Surprisingly, pre-methylated adenine can be erroneously incorporated into DNA as misincorporated N6-methyladenine (i6mdA) via DNA polymerase-mediated replication. Despite pr6mdA and i6mdA sharing identical chemical structures, their biological functions diverge significantly, presenting a substantial challenge in distinguishing between the two. Here, for the first-time, it is exploited that the adenosine deaminase-like (Adal) protein and a corresponding activity-null mutant to construct an Adal lentivirus toolkit. With this newly designed toolkit, both pr6mdA and i6mdA can be identified and

SUBMITTER: Liang Z 

PROVIDER: S-EPMC11425204 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature