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The single-cell transcriptome of mTECs and CD4⁺ thymocytes under adhesion revealed heterogeneity of mTECs and a network controlled by Aire and lncRNAs.

ABSTRACT: To further understand the impact of deficiency of the autoimmune regulator (Aire) gene during the adhesion of medullary thymic epithelial cells (mTECs) to thymocytes, we sequenced single-cell libraries (scRNA-seq) obtained from Aire wild-type (WT) (Aire^wt/wt ) or Aire-deficient (Aire^wt/mut ) mTECs cocultured with WT single-positive (SP) CD4⁺ thymocytes. Although the libraries differed in their mRNA and long noncoding RNA (lncRNA) profiles, indicating that mTECs were heterogeneous in terms of their transcriptome, UMAP clustering revealed that both mTEC lines expressed their specific markers, i.e., Epcam, Itgb4, Itga6, and Casp3 in resting mTECs and Ccna2, Pbk, and Birc5 in proliferative mT

SUBMITTER: Monteiro CJ

PROVIDER: S-EPMC11425717 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Dataset Information

The single-cell transcriptome of mTECs and CD4⁺ thymocytes under adhesion revealed heterogeneity of mTECs and a network controlled by Aire and lncRNAs.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

The single-cell transcriptome of mTECs and CD4+ thymocytes under adhesion revealed heterogeneity of mTECs and a network controlled by Aire and lncRNAs.

OmicsDI is part of the ELIXIR infrastructure

Tweets

The single-cell transcriptome of mTECs and CD4⁺ thymocytes under adhesion revealed heterogeneity of mTECs and a network controlled by Aire and lncRNAs.