Nanoplasmonic Single-Tumoroid Microarray for Real-Time Secretion Analysis.

ABSTRACT: Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment of their functional behavior, particularly protein secretion, is of significant importance to provide comprehensive insights. Here, a label-free spectroscopic imaging platform is presented with advanced integrated optofluidic nanoplasmonic biosensor that enables real-time secretion analysis from single tumoroids. A novel two-layer microwell design isolates tumoroids, preventing signal interference, and the microarray configuration allows concurrent analysis of multiple tumoroids. The dual imaging capability combining time-lapse plasmonic s

SUBMITTER: Liu YC 

PROVIDER: S-EPMC11425908 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature