Characterization of Mycobacterium smegmatis Glutaminase-Free Asparaginase (MSMEG_3173).

ABSTRACT: l-asparaginase is an enzyme catalyzing the hydrolysis of l-asparagine into l-aspartate and ammonia, which is of great therapeutic importance in tumor treatment. However, commercially available enzymes are associated with adverse effects, and searching for a new l-asparaginase with better pharmaceutical properties was the aim of this work. The coding sequence for Mycobacterium smegmatisl-asparaginase (MsA) was cloned and expressed. The recombinant protein showed high activity toward l-asparagine, whereas none was detected for l-glutamine. The enzymatic properties (K _m = 1.403 ± 0.24 mM and k _cat = 708.1 ± 25.05 s^-1) indicate that the enzyme would be functional within the expected blood l-asparagine concentration, with good activity, as s

SUBMITTER: Rezende Correa P 

PROVIDER: S-EPMC11425952 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature