Transcriptomic signatures during normothermic liver machine perfusion correspond with graft quality and predict the early graft function.

ABSTRACT:

Background

A better understanding of the molecular events during liver normothermic machine perfusion (NMP) is warranted to develop a data-based approach for the identification of biomarkers representative of graft quality and posttransplant outcome. We analysed the dynamic transcriptional changes during NMP and linked them to clinical and biochemical parameters.

Methods

50 livers subjected to NMP for up to 24 h were enrolled. Bulk RNA sequencing was performed in serial biopsies collected pre and during NMP, and after reperfusion. Perfusate was sampled to monitor liver function. qPCR and immunohistochemistry were performed to validate findings. Molecular profiles were compared between transplanted and non-transplanted livers, and livers with and without early allograft dysfu

SUBMITTER: Hautz T 

PROVIDER: S-EPMC11426134 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature