Mutant IDH Modulates Suppressive Myeloid Populations in Malignant Glioma.

ABSTRACT:

Purpose

Mutations in the isocitrate dehydrogenase (IDH) genes IDH1 and IDH2 have critical diagnostic and prognostic significance in diffuse gliomas. Neomorphic mutant IDH activity has been previously implicated in T-cell suppression; however, the effects of IDH mutations on intratumoral myeloid populations remain underexplored. In this study, we investigate the influence of IDH status on the myeloid compartment using human glioma specimens and preclinical models.

Experimental design

We performed RNA sequencing and quantitative immunofluorescence on newly diagnosed, treatment-naive IDH-mutant grade 4 astrocytoma and IDH-wild-type (IDH-WT) glioblastoma (GBM) specimens. We also generated a syngeneic murine model, comparing transcriptomic and cell-level changes in paired isogeni

SUBMITTER: Grewal EP 

PROVIDER: S-EPMC11426330 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature