Physiology of trans-translation deficiency in Bacillus subtilis - a comparative proteomics study.

ABSTRACT: trans-Translation is the most effective ribosome rescue system known in bacteria. While it is essential in some bacteria, Bacillus subtilis possesses two additional alternative ribosome rescue mechanisms that require the proteins BrfA or RqcH. To investigate the physiology of trans-translation deficiency in the model organism B. subtilis, we compared the proteomes of B. subtilis 168 and a ΔssrA mutant in the mid-log phase using gel-free label-free quantitative proteomics. In chemically defined medium, the growth rate of the ssrA deletion mutant was 20% lower than that of B. subtilis 168. An ³⁵ S-methionine incorporation assay demonstrated that protein synthesis rates were also lower in the ΔssrA strain. Alternative rescue factors were not detected. Among the 34 proteins overrepr

SUBMITTER: Vazquez-Hernandez M 

PROVIDER: S-EPMC11426337 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature