Serum-deprivation response of ARPE-19 cells; expression patterns relevant to age-related macular degeneration.

ABSTRACT: ARPE-19 cells are derived from adult human retinal pigment epithelium (RPE). The response of these cells to the stress of serum deprivation mimics some important processes relevant to age-related macular degeneration (AMD). Here we extend the characterization of this response using RNASeq and EGSEA gene set analysis of ARPE-19 cells over nine days of serum deprivation. This experiment confirmed the up-regulation of cholesterol and lipid-associated pathways that increase cholesterol levels in these cells. The gene expression analysis also identified other pathways relevant to AMD progression. There were significant changes in extracellular matrix gene expression, notably a switch from expression of collagen IV, a key component of Bruch's membrane (part of the blood-retina barrier), to expre

SUBMITTER: Peterson KM 

PROVIDER: S-EPMC11426544 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature