Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion.

ABSTRACT: The identification of tumor capsular invasion as a sign of malignancy is currently employed in traditional histopathology routines for thyroid nodules. However, its limitations are associated with the assessment criteria for invasion, which often lead to disagreements among observers. The aim of this paper is to introduce a widefield imaging technique combined with quantitative collagen analysis to identify areas of capsular invasion in thyroid neoplasms. In this study, we introduce the application of widefield polarization-resolved second harmonic generation microscopy for imaging entire thyroid nodule sections on histological slides. We employ a cylindrical collagen model to extract parameters associated with the ultrastructure and orientation of collagen within the entire capsule of the

SUBMITTER: Eftimie LG 

PROVIDER: S-EPMC11427203 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature