Genetically programmed synthetic cells for thermo-responsive protein synthesis and cargo release.

ABSTRACT: Synthetic cells containing genetic programs and protein expression machinery are increasingly recognized as powerful counterparts to engineered living cells in the context of biotechnology, therapeutics and cellular modelling. So far, genetic regulation of synthetic cell activity has been largely confined to chemical stimuli; to unlock their potential in applied settings, engineering stimuli-responsive synthetic cells under genetic regulation is imperative. Here we report the development of temperature-sensitive synthetic cells that control protein production by exploiting heat-responsive mRNA elements. This is achieved by combining RNA thermometer technology, cell-free protein expression and vesicle-based synthetic cell design to create cell-sized capsules able to initiate synthesis of bo

SUBMITTER: Monck C 

PROVIDER: S-EPMC11427347 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature